ABSTRACT
Background: Some antidepressant drugs can promote neuronal cell proliferation in vitro as well as hippocampal neurogenesis in human and animal models. Furthermore, adipose tissue is an available source of adult stem cells with the ability to differentiate in to multiple lineages. Therefore, human Adipose-Derived Stem Cells [hADSCs] may be a suitable source for regenerative medical applications. Since there is no evidence for the effect of Paroxetine as the most commonly prescribed antidepressant drug for neurogenic potential of hADSCs, an attempt was made to determine the effect of Paroxetine on proliferation and neural differentiation of hADSCs
Methods: ADSCs were isolated from human abdominal fat. These cells differentiated to neuron-like cells and were treated with Paroxetine. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide [MTT] assay and immunofluorescence technique were used for assessment of cell proliferation and neurogenic differentiation potential of induced cells, respectively
Results: MTT assay analysis showed that Paroxetine significantly increased the proliferation rate of induced hADSCs [p<0.05], while immunofluorescent staining indicated that Paroxetine treatment during neurogenic differentiation could enhance the mean percentage of Nestin and MAP2 [Microtubule-associated protein-2] positive cells but the mean percentage of GFAP [Glial acidic fibrillary protein] positive cells significantly decreased relative to control group [p<0.05]
Conclusion: Our results provide evidence that Paroxetine can promote proliferation and differentiation rate during neurogenic differentiation of hADSCs. Moreover, Paroxetine can reduce gliogenesis of induced hADSCs during neurogenic differentiation
ABSTRACT
Due to the restricted potential of neural stem cells for regeneration of central nervous system [CNS] after injury, providing an alternative source for neural stem cells is essential. Adipose derived stem cells [ADSCs] are multipotent cells with properties suitable for tissue engineering. In addition, alginate hydrogel is a biocompatible polysaccharide polymer that has been used to encapsulate many types of cells. The aim of this study was to assess the proliferation rate and level of expression of neural markers; NESTIN, glial fibrillary acidic protein [GFAP] and microtubule-associated protein 2 [MAP2] in encapsulated human ADSCs [hADSCs] 10 and14 days after neural induction. In this experimental study, ADSCs isolated from human were cultured in neural induction media and seeded into alginate hydrogel. The rate of proliferation and differentiation of encapsulated cells were evaluated by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide [MTT] assay, immunocytoflourescent and real-time reverse transcriptase polymerase chain reaction [RT-PCR] analyzes 10 and 14 days after induction. The rate of proliferation of encapsulated cells was not significantly changed with time passage. The expression of NESTIN and GFAP significantly decreased on day 14 relative to day 10 [P<0.001] but MAP2 expression was increased. Alginate hydrogel can promote the neural differentiation of encapsulated hADSCs with time passage